Tumor Biology

Research Article

Reduced Expression of 15-Lipoxygenase 2 in Human Head and Neck Carcinomas

Wang D.a · Chen S.a · Feng Y.a · Yang Q.a · Campbell B.H.b · Tang X.c · Campbell W.B.c

Author affiliations

Departments of aRadiation Oncology, bOtolaryngology, cPharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisc., USA

Keywords: 15-LipoxygenaseHead and neck cancerCarcinogenesis15-Hydroxyeicosatetraenoic acid

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Tumor Biol 2006;27:261–273
https://doi.org/10.1159/000094761

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Article / Publication Details

First-Page Preview
Abstract of Research Article

Received: September 30, 2005
Accepted: January 31, 2006
Published online: August 23, 2006
Issue release date: August 2006

Number of Print Pages: 13
Number of Figures: 9
Number of Tables: 1

ISSN: 1010-4283 (Print)
eISSN: 1423-0380 (Online)

For additional information: https://www.karger.com/TBI

Abstract

Nonsteroidal anti-inflammatory drugs (NSAIDs) have demonstrated cancer chemoprevention effects associated with their ability to modulate polyunsaturated fatty acid metabolism. In the present study, we report a significant reduction of 15-lipoxygenase 2 (15-LOX-2) in seven carcinoma cell lines of the human head and neck when compared with normal primary cultured keratinocytes, and 18 primary head and neck squamous cell carcinomas (HNSCC) when compared with matched normal mucosa. 15-LOX-2 is mainly expressed in the mature cells of the benign squamous epithelium, but not in the basal layer cells of benign epithelium, suggesting a role of 15-LOX-2 in cell differentiation. We further found that 15-lipoxygenase activity was reduced in carcinoma cells when compared with normal primary cultured keratinocytes. When the effects of NSAIDs were examined on cell proliferation and regulation of 15-LOX-2 in the carcinoma cells, NS398 treatment resulted in significant growth inhibition associated with upregulation of 15-LOX-2 and its major metabolite 15-S-HETE. Finally, restoration of 15-LOX-2 expression into these carcinoma cells significantly inhibited cell proliferation. Our results demonstrate that 15-LOX-2 expression is significantly reduced and this reduction may promote proliferation in human head and neck carcinoma. 15-LOX-2 may be a possible biomarker in human head and neck malignancy.

© 2006 S. Karger AG, Basel



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Article / Publication Details

First-Page Preview
Abstract of Research Article

Received: September 30, 2005
Accepted: January 31, 2006
Published online: August 23, 2006
Issue release date: August 2006

Number of Print Pages: 13
Number of Figures: 9
Number of Tables: 1

ISSN: 1010-4283 (Print)
eISSN: 1423-0380 (Online)

For additional information: https://www.karger.com/TBI

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