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Pharmacology

Iron(III) Chloride Hexahydrate Does Not Enhance Methotrexate Cytotoxicity on Saccharomyces cerevisiae

Ruiz-Gómez M.J. · Martínez-Morillo M.

Author affiliations

Laboratory of Radiobiology, Department of Radiology and Physical Medicine, Faculty of Medicine, University of Malaga, Teatinos s/n, Malaga, Spain

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Chemotherapy 2006;52:226–230

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Article / Publication Details

First-Page Preview
Abstract of Pharmacology

Received: November 09, 2004
Accepted: September 06, 2005
Published online: August 25, 2006
Issue release date: August 2006

Number of Print Pages: 5
Number of Figures: 3
Number of Tables: 1

ISSN: 0009-3157 (Print)
eISSN: 1421-9794 (Online)

For additional information: https://www.karger.com/CHE

Abstract

Methotrexate is a potent inhibitor of dihydropholate reductase that has been used as effective antineoplastic treatment due to its capacity to inhibit cell growth. In a previous work published in Bioelectrochemistry 2003;60:81—86, we reported a statistically significant increment of 40.1 and 29.4% in methotrexate potency when MCF-7 breast cancer cells were exposed simultaneously to iron(III) chloride hexahydrate (FeCl3·6H2O) and methotrexate. The aim of this study was to investigate whether iron(III) could produce, on a Saccharomyces cerevisiae wild-type strain, alterations on methotrexate potency by the drop test survival assay and proliferation studies measured after 24 and 96 h of exposure. The data presented in the current report indicate that FeCl3·6H2O (1, 10, 100 and 500 µg/ml) does not induce modulation of the action of methotrexate (10, 100 and 500 µg/ml) in S. cerevisiae yeast cells when they are exposed simultaneously for 24 and 96 h.

© 2006 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Pharmacology

Received: November 09, 2004
Accepted: September 06, 2005
Published online: August 25, 2006
Issue release date: August 2006

Number of Print Pages: 5
Number of Figures: 3
Number of Tables: 1

ISSN: 0009-3157 (Print)
eISSN: 1421-9794 (Online)

For additional information: https://www.karger.com/CHE


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