Nephron Experimental Nephrology
Original Paper
Evidence that the Mouse Osteocalcin-Related Gene Does Not Encode NephrocalcinPetrucci M. · Paquette Y. · Leblond F.A. · Pichette V. · Bonnardeaux A.Centre de Recherche Guy-Bernier, Hôpital Maisonneuve-Rosemont, Montréal, Canada
|
|
Log in to MyKarger to check if you already have access to this content.
KAB
Buy a Karger Article Bundle (KAB) and profit from a discount!
If you would like to redeem your KAB credit, please log in.
Save over 20% compared to the individual article price.
Subscribe
For eJournal Archive and eJournal Backfiles information please contact Karger service
Article / Publication Details
Received: September 22, 2005
Accepted: January 25, 2006
Published online: August 11, 2006
Issue release date: November 2006
Number of Print Pages: 1
Number of Figures: 3
Number of Tables: 1
eISSN: 1660-2129 (Online)
For additional information: https://www.karger.com/NEE
Abstract
Background/Aims: The osteocalcin-related gene (ORG) is a mouse-specific member of the osteocalcin gene cluster predicted to encode a γ-carboxyglutamic acid-rich protein. ORG mRNA has been predicted to encode nephrocalcin and shown to be expressed in the kidney where it could serve as an important crystallization inhibitor. To determine whether ORG encodes mouse nephrocalcin, we investigated its in vivo and in vitro expression. Methods: We expressed fluorescent fusion ORG proteins in kidney cell lines and generated transgenic mice expressing enhanced green fluorescent protein under the control of the ORG promoter. Results:ORG mRNA was shown to be expressed in mouse kidneys and in a variety of other tissues. Fusion constructs transfected in opossum kidney cells demonstrated integrity of the open reading frame with the presence of a protein of the expected molecular weight. However, kidneys from transgenic mice carrying the enhanced green fluorescent protein gene under the control of the ORG promoter (5.8 kb fragment) demonstrated no expression of the transgene in kidneys or other tissues. Conclusion: We conclude that ORG, the third gene of the mouse osteocalcin gene cluster is silent and unlikely to play a major role in mouse renal physiology.
© 2006 S. Karger AG, Basel
Related Articles:
References
- Coe FL, Evan A, Worcester E: Kidney stone disease. J Clin Invest 2005;115:2598–2608.
- Poser JW, et al: Isolation and sequence of the vitamin K-dependent protein from human bone. Undercarboxylation of the first glutamic acid residue. J Biol Chem 1980;255:8685–8691.
- Ducy P, et al: Increased bone formation in osteocalcin-deficient mice. Nature 1996;382:448–452.
- Desbois C, Hogue DA, Karsenty G: The mouse osteocalcin gene cluster contains three genes with two separate spatial and temporal patterns of expression. J Biol Chem 1994;269:1183–1190.
- Sato M, Tada N: Preferential expression of osteocalcin-related protein mRNA in gonadal tissues of male mice. Biochem Biophys Res Commun 1995;215:412–421.
- Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680–685.
- Towbin H, Staehelin T, Gordon J: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979;76:4350–4354.
-
Hogan B, et al: Manipulating the Mouse Embryo, ed 2. Plainview, New York, C.S.H.L. Press, 1994.
- Tabernero C, et al: Identification of an RNA sequence within an intracisternal-A particle element able to replace Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1. J Virol 1997;71:95–101.
- Kuff EL, Lueders KK: The intracisternal A-particle gene family: structure and functional aspects. Adv Cancer Res 1988;51:183–276.
- Heidmann O, Heidmann T: Retrotransposition of a mouse IAP sequence tagged with an indicator gene. Cell 1991;64:159–170.
- Kuff EL: Intracisternal A particles in mouse neoplasia. Cancer Cells 1990;2:398–400.
- Mietz JA, Kuff EL: Tissue and strain-specific patterns of endogenous proviral hypomethylation analyzed by two-dimensional gel electrophoresis. Proc Natl Acad Sci USA 1990;87:2269–2273.
- Yoder JA, Walsh CP, Bestor TH: Cytosine methylation and the ecology of intragenomic parasites. Trends Genet 1997;13:335–340.
- Gwynn B, et al: Intracisternal A-particle element transposition into the murine beta-glucuronidase gene correlates with loss of enzyme activity: a new model for beta-glucuronidase deficiency in the C3H mouse. Mol Cell Biol 1998;18:6474–6481.
- Nigumann P, et al: Many human genes are transcribed from the antisense promoter of L1 retrotransposon. Genomics 2002;79:628–634.
- Michaud EJ, et al: Differential expression of a new dominant agouti allele (Aiapy) is correlated with methylation state and is influenced by parental lineage. Genes Dev 1994:8:1463–1472.
- Barbot W, et al: Epigenetic regulation of an IAP retrotransposon in the aging mouse: progressive demethylation and de-silencing of the element by its repetitive induction. Nucleic Acids Res 2002;30:2365–2373.
Article / Publication Details
Received: September 22, 2005
Accepted: January 25, 2006
Published online: August 11, 2006
Issue release date: November 2006
Number of Print Pages: 1
Number of Figures: 3
Number of Tables: 1
eISSN: 1660-2129 (Online)
For additional information: https://www.karger.com/NEE
Copyright / Drug Dosage / Disclaimer
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
Get Permission
PubMed ID
