Cellular Physiology and Biochemistry
Original Paper
Electrophysiological Demonstration of Voltage- Activated H+ Channels in Bovine Articular ChondrocytesSánchez J.C.1 · Powell T.2 · Staines H.M.3 · Wilkins R.J.21Universidad Tecnológica de Pereira, Departamento de Ciencias Básicas - Facultad de Ciencias de la
Salud, A.A. 97 - La Julita, Pereira - Risaralda – Colombia, 2Department of Physiology, Anatomy and
Genetics, Parks Road, University of Oxford, 3Centre for Infection, Division of Cellular and Molecular
Medicine, St George’s, University of London
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Abstract
Matrix synthesis by articular chondrocytes is sensitive to changes in intracellular pH (pHi), so characterising the membrane transport pathways that determine pHi is important for understanding how chondrocytes regulate the turnover of cartilage matrix. In the present study, the whole-cell patch-clamp technique has been employed to demonstrate the operation of voltageactivated H+ channels (VAHC) in bovine articular chondrocytes. Using solutions designed to minimise the contribution of ions other than H+, the application of step voltage-protocols elicited whole-cell currents. These currents were slow activating, observed only in the outward direction, dependent on both extracellular pH (pHo) and pHi, and inhibited by Zn2+. The reversal potential values, measured by tail current analysis, over a range of different pHo and pHi values, were in good agreement with predicted values for membrane channels having a high selectivity for protons. The results presented here are consistent with the operation of VAHC in articular chondrocytes.
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Article / Publication Details
Published online: August 15, 2006
Issue release date: January 2006
Number of Print Pages: 6
Number of Figures: 0
Number of Tables: 0
ISSN: 1015-8987 (Print)
eISSN: 1421-9778 (Online)
For additional information: https://www.karger.com/CPB
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