International Archives of Allergy and Immunology
Original Paper
High Vascular Endothelial Growth Factor Levels in NZW Mice Do Not Correlate with Collagen Deposition in Allergic AsthmaVasquez-Pinto L.M.C.a · Landgraf R.G.a · Bozza P.T.b · Jancar S.aaDepartment of Immunology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, and bDepartment of Physiology and Pharmacodinamics, Institute Oswaldo Cruz, Rio de Janeiro, Brazil
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Article / Publication Details
Received: January 31, 2006
Accepted: June 20, 2006
Published online: September 29, 2006
Issue release date: December 2006
Number of Print Pages: 9
Number of Figures: 6
Number of Tables: 0
ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)
For additional information: https://www.karger.com/IAA
Abstract
Background: Eosinophils contribute to the early features of allergic lung inflammation through the generation and release of a plethora of mediators. Eosinophil peroxidase (EPO) is one of the eosinophil granule proteins involved in the early response, but its participation in airway remodeling is not established. The present study addressed this question comparing an EPO-deficient mouse strain (NZW) with BALB/c and C57Bl/c strains. Methods: Mice were immunized with ovalbumin/alum, challenged twice with ovalbumin aerosol, and lung responses were measured at day 22 or 28. Collagen, mucus and eosinophils were determined in lung sections stained with picrosirius, periodic acid-Schiff or hematoxylin-eosin; transforming growth factor-β and vascular endothelial growth factor were determined by ELISA, lipid bodies by enumeration in osmium-stained eosinophils, and airway reactivity to methacholine in isolated lung preparations. Results: NZW mice showed significantly less collagen around bronchi and blood vessels, less mucus and less eosinophils around bronchi. Eosinophil lipid body formation and airway hyperreactivity were comparable among strains. Levels of transforming growth factor-β were also comparable; however, the NZW mice showed much higher levels of vascular endothelial growth factor, even under basal conditions. Conclusions: In allergic lung inflammation, the combination of EPO deficiency and overexpression of VEGF found in NZW mice is associated with less collagen deposition, less mucus and reduced tissue eosinophilia. Eosinophil activation and airway hyperreactivity in NZW mice were similar to the other strains.
© 2007 S. Karger AG, Basel
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Article / Publication Details
Received: January 31, 2006
Accepted: June 20, 2006
Published online: September 29, 2006
Issue release date: December 2006
Number of Print Pages: 9
Number of Figures: 6
Number of Tables: 0
ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)
For additional information: https://www.karger.com/IAA
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