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Laboratory/Clinical Translational Research

Mutational Analysis of AKT1, AKT2 and AKT3 Genes in Common Human Carcinomas

Soung Y.H. · Lee J.W. · Nam S.W. · Lee J.Y. · Yoo N.J. · Lee S.H.

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Department of Pathology, College of Medicine, Catholic University of Korea, Seoul, Korea

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Oncology 2006;70:285–289

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Article / Publication Details

First-Page Preview
Abstract of Laboratory/Clinical Translational Research

Received: March 23, 2006
Accepted: August 05, 2006
Published online: October 31, 2006
Issue release date: October 2006

Number of Print Pages: 5
Number of Figures: 1
Number of Tables: 2

ISSN: 0030-2414 (Print)
eISSN: 1423-0232 (Online)

For additional information: https://www.karger.com/OCL

Abstract

Objective: Mounting evidence indicates that alterations in AKT proteins play an important role in the pathogenesis of cancer. The objective of this study was to see whether common human carcinomas harbor AKT mutations that might contribute to the development of cancer. Methods: We performed mutational analysis of the kinase domains of AKT1–AKT3 by a single-strand conformation polymorphism assay in 294 carcinoma tissues from the stomach, lung, colon and breast. Results: Overall, we detected three somatic mutations in AKT2, but no mutations in AKT1 or AKT3 in the 294 cancer tissues. The AKT2 mutations were detected in 1 of 51 gastric carcinomas (2.0%) and 2 of 79 lung carcinomas (2.5%). AKT2 mutations consisted of one missense mutation and 2 splice site mutations in the intron. We simultaneously analyzed somatic mutations in EGFR, ERBB2, K-RAS, PIK3CA and BRAF genes in the 3 samples with the AKT2 mutations, and found a lung adenocarcinoma with the AKT2 missense mutation harbored an EGFR mutation. Conclusion: This study demonstrated that somatic mutations in the kinase domain of AKT2 occur in a small fraction of common human cancers, and suggested that alterations in the AKT2-mediated signaling pathway by AKT2 mutation could contribute to the development of some cases of human cancers.

© 2006 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Laboratory/Clinical Translational Research

Received: March 23, 2006
Accepted: August 05, 2006
Published online: October 31, 2006
Issue release date: October 2006

Number of Print Pages: 5
Number of Figures: 1
Number of Tables: 2

ISSN: 0030-2414 (Print)
eISSN: 1423-0232 (Online)

For additional information: https://www.karger.com/OCL


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