Nephron Experimental Nephrology
Original Paper
Reduction of Renal Fibrosis as a Result of Liposome Encapsulated Clodronate Induced Macrophage Depletion after Unilateral Ureteral Obstruction in RatsSung S.A.a · Jo S.K.b · Cho W.Y.b · Won N.H.c · Kim H.K.baDivision of Nephrology, Eulji Hospital, and Departments of bInternal Medicine and cPathology, Korea University Hospital, Seoul, Korea
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Article / Publication Details
Received: January 11, 2006
Accepted: December 06, 2006
Published online: November 09, 2006
Issue release date: December 2006
Number of Print Pages: 1
Number of Figures: 8
Number of Tables: 0
eISSN: 1660-2129 (Online)
For additional information: https://www.karger.com/NEE
Abstract
Background/Aim: Macrophages have been thought to play a role in renal tubulointerstitial fibrosis; recent reports have demonstrated an antifibrotic effect of macrophages in late-stage renal fibrosis. Liposome-encapsulated clodronate (LC) produces a selective and systemic depletion of phagocytic macrophages in vivo. To study the role of initial infiltrating macrophages in renal fibrosis, we compared the effects of pretreatment with LC and a liposome vehicle for control of the severity of renal fibrosis in a unilateral ureteral obstruction (UUO) rat model. Methods: One day after a single intravenous injection of LC or liposome vehicle, the rats underwent UUO. Following 1, 5, and 14 days, the kidneys were examined to evaluate macrophage infiltration and renal fibrosis. Results: LC depleted macrophages systemically and reduced renal fibrosis associated with UUO; this beneficial effect was accompanied by a decrease of transforming growth factor beta mRNA expression. The osteopontin expression was also reduced by pretreatment with LC. Conclusion: Initial interstitial infiltration of macrophages contributes to tubulointerstitial fibrosis in UUO.
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Article / Publication Details
Received: January 11, 2006
Accepted: December 06, 2006
Published online: November 09, 2006
Issue release date: December 2006
Number of Print Pages: 1
Number of Figures: 8
Number of Tables: 0
eISSN: 1660-2129 (Online)
For additional information: https://www.karger.com/NEE
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