Cellular Physiology and Biochemistry

Original Paper

Free Access

Vectorial Bicarbonate Transport by Capan-1 Cells: a Model for Human Pancreatic Ductal Secretion

Szucs A.1 · Demeter I.1 · Burghardt B.1 · Ovari G.1 · Case R.M.2 · Steward M.C.2 · Varga G.1

Author affiliations

1Molecular Oral Biology Research Group, Department of Oral Biology, Semmelweis University and Hungarian Academy of Sciences, Budapest, 2Faculty of Life Sciences, University of Manchester

Corresponding Author

Professor Gabor Varga

Department of Oral Biology, Semmelweis University

Nagyvarad ter 4, H-1089 Budapest (Hungary)

Tel. +36-1-210-4415, Fax: +36-1-210-4421

E-Mail varga-g@net.sote.hu

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Abstract

Human pancreatic ducts secrete a bicarbonate-rich fluid but our knowledge of the secretory process is based mainly on studies of animal models. Our aim was to determine whether the HCO3- transport mechanisms in a human ductal cell line are similar to those previously identified in guinea-pig pancreatic ducts. Intracellular pH was measured by microfluorometry in Capan-1 cell monolayers grown on permeable filters and loaded with BCECF. Epithelial polarization was assessed by immunolocalization of occludin. Expression of mRNA for key electrolyte transporters and receptors was evaluated by RT-PCR. Capan-1 cells grown on permeable supports formed confluent, polarized monolayers with well developed tight junctions. The recovery of pHi from an acid load, induced by a short NH4+ pulse, was mediated by Na+-dependent transporters located exclusively at the basolateral membrane. One was independent of HCO3- and blocked by EIPA (probably NHE1) while the other was HCO3--dependent and blocked by H2DIDS (probably pNBC1). Changes in pHi following blockade of basolateral HCO3- accumulation confirmed that the cells achieve vectorial HCO3- secretion. Dose-dependent increases in HCO3- secretion were observed in response to stimulation of both secretin and VPAC receptors. ATP and UTP applied to the apical membrane stimulated HCO3- secretion but were inhibitory when applied to the basolateral membrane. HCO3- secretion in guinea-pig ducts and Capan-1 cell monolayers share many common features, suggesting that the latter is an excellent model for studies of human pancreatic HCO3- secretion.




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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: November 29, 2006
Issue release date: January 2006

Number of Print Pages: 12
Number of Figures: 0
Number of Tables: 0

ISSN: 1015-8987 (Print)
eISSN: 1421-9778 (Online)

For additional information: https://www.karger.com/CPB


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