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Original Paper

Full-Length Sequences of Two Hepatitis E Virus Isolates Representing an Eastern China-Indigenous Subgroup of Genotype 4

Koike M.a · Takahashi K.b · Mishiro S.b · Matsui A.a · Inao M.a · Nagoshi S.a · Ohno A.a · Mochida S.a · Fujiwara K.c

Author affiliations

aDivision of Gastroenterology and Hepatology, Department of Internal Medicine, Saitama Medical University, Saitama, bDepartment of Medical Sciences, Toshiba General Hospital, Tokyo, and cYokohama Rosai Hospital, Yokohama, Japan

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Intervirology 2007;50:181–189

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: September 27, 2006
Published online: January 26, 2007
Issue release date: April 2007

Number of Print Pages: 9
Number of Figures: 4
Number of Tables: 2

ISSN: 0300-5526 (Print)
eISSN: 1423-0100 (Online)

For additional information: https://www.karger.com/INT

Abstract

Although the majority of hepatitis E virus (HEV) infections in Japan are ‘domestic’ due to the presence of indigenous strains, there are still ‘imported’ cases as well. Among 83 patients with non-A, non-B and non-C acute liver diseases admitted to Saitama Medical University Hospital, 7 (8.4%) were positive for serum HEV-RNA, of whom 2 had a recent history of traveling to China, one to Xian and another to Shanghai. We determined the full-genome sequences of HEV from these 2 patients (isolate names are JKO-ChiSai98c and JYI-ChiSai01c, genotype 4 in both) for phylogenetic analyses. Initially, when compared only to the 13 full-genome sequences of genotype 4 so far reported, our 2 isolates were thought to be novel strains because they showed a significant genetic difference from the sequences known to date. However, when we included a set of short sequences (150 nt) recently reported from China in the comparison, we found that our 2 isolates represent a subgroup of genotype 4, which seems to be restricted to eastern China. In conclusion, the 2 HEV isolates reported here could serve as full-genome prototypes for an eastern China-indigenous subgroup of the genotype 4 HEV.

© 2007 S. Karger AG, Basel


References

  1. Balayan MS, Andjaparidze AG, Savinskaya SS, Ketiladze ES, Braginsky DM, Savinov AP, Poleschuk VF: Evidence for a virus in non-A, non-B hepatitis transmitted via the fecal-oral route. Intervirology 1983;20:23–31.
  2. Schrader GG, Dawson GJ, Erker JC, Kwo PY, Knigge MF, Smalley DL, Rosenblatt JE, Desai SM, Mushahwar IK: The sequence and phylogenetic analysis of a novel hepatitis E virus isolated from a patient with acute hepatitis reported in the United States. J Gen Virol 1998;79:449–456.
  3. Schlauder GG, Desai SM, Zanetti AR, Tassopoulos NC, Mushahwar IK: Novel hepatitis E virus (HEV) isolates from Europe: evidence for additional genotypes of HEV. J Med Virol 1999;57:243–251.
  4. Zanetti AR, Schlauder GG, Romano L, Tanzi E, Fabris P, Dawson GJ, Mushahwar IK: Identification of a novel variant of hepatitis E virus in Italy. J Med Virol 1999;57:356–360.
  5. Mansuy JM, Peron JM, Abravanel F, Poirson H, Dubois M, Miedouge M, Vischi F, Alric L, Vinel JP, Izopet J: Hepatitis E in the south west of France in individuals who have never visited an endemic area. J Med Virol 2004;74:419–424.
  6. Takahashi K, Iwata K, Watanabe N, Hatahara T, Ohta Y, Baba K, Mishiro S: Full-genome nucleotide sequence of a hepatitis E virus strain that may be indigenous to Japan. Virology 2001;287:9–12.
  7. Takahashi K, Kang JH, Ohnishi S, Hino K, Mishiro S: Genetic heterogeneity of hepatitis E virus recovered from Japanese patients with acute sporadic hepatitis. J Infect Dis 2002;185:1342–1345.
  8. Mizuo H, Suzuki K, Takikawa Y, Sugai Y, Tokita H, Akahane Y, Itoh K, Gotanda Y, Takahashi M, Nishizawa T, Okamoto H: Polyphyletic strains of hepatitis E virus are responsible for sporadic cases of acute hepatitis in Japan. J Clin Microbiol 2002;40:3209–3218.
  9. Meng XJ, Purcell RH, Halbur PG, Lehman JR, Webb DM, Tsareva TS, Haynes JS, Thacker BJ, Emerson SU: A novel virus in swine is closely related to the human hepatitis E virus. Proc Natl Acad Sci USA 1997;94:9860–9865.
  10. Tei S, Kitajima N, Takahashi K, Mishiro S: Zoonotic transmission of hepatitis E virus from deer to human beings. Lancet 2003;362:371–373.
  11. Matsuda H, Okada K, Takahashi K, Mishiro S: Severe hepatitis E virus infection after ingestion of uncooked liver from a wild boar. J Infect Dis 2003;188:944.
  12. Yazaki Y, Mizuo H, Takahashi M, Nishizawa T, Sasaki N, Gotanda Y, Okamoto H: Sporadic acute or fulminant hepatitis E in Hokkaido, Japan, may be food-borne, as suggested by the presence of hepatitis E virus in pig liver as food. J Gen Virol 2003;84:2351–2357.
  13. Mochida S, Fujiwara K: Symposium on clinical aspects in hepatitis virus infection. 2. Recent advances in acute and fulminant hepatitis in Japan. Intern Med 2001;40:175–177.
  14. Takahashi K, Kang JH, Ohnishi S, Hino K, Miyakawa H, Miyakawa Y, Maekubo H, Mishiro S: Full-length sequences of six hepatitis E virus isolates of genotypes III and IV from patients with sporadic acute or fulminant hepatitis in Japan. Intervirology 2003;46:308–318.
  15. Tam AW, Smith MM, Guerra ME, Hunang CC, Bradley DW, Fry KE, Reyes GR: Hepatitis E virus (HEV): Molecular cloning and sequencing of the full-length viral genome. Virology 1991;185:120–131.
  16. Felsenstein J: Confidence limits on phylogenesis: an approach using the bootstrap. Evolution 1985;39:783–791.
    External Resources
  17. Gojobori T, Ishii K, Nei M: Estimation of average number of nucleotide substitutions when the rate of substitution varies. J Mol Evol 1982;28:414–423.
    External Resources
  18. Saitou N, Nei M: The neighbor-joining methods: a new for reconstruction phylogenetic trees. Mol Biol Evol 1987;4:406–425.
  19. Prager EM, Wilson AC: Construction of phylogenetic trees for proteins and nucleic acids: empirical evaluation of alternative matrix methods. J Mol Evol 1978;11:129–142.
  20. Irshad M: Hepatitis E virus: an update on its molecular, clinical and epidemiological characteristics. Intervirology 1999;42:252–262.
  21. Aggarwal R, Krawczynski K: Hepatitis E: an overview and recent advances in clinical and laboratory research. J Gastroenterol Hepatol 2000;15:9–20.
  22. Wang Y, Zhang H, Li Z, Gu W, Lan H, Hao W, Ling R, Li H, Harrison TJ: Detection of sporadic cases of hepatitis E virus (HEV) infection in China using immunoassays based on recombinant open reading frame 2 and 3 polypeptides from HEV genotype 4. J Clin Microbiol 2001;39:4370–4379.
  23. Takahashi K, Toyota J, Karino Y, Kang J-H, Maekubo H, Abe N, Mishiro S: Estimation of the mutation rate of hepatitis E virus based on a set of closely related 7.5-year-apart isolates from Sapporo, Japan. Hepatol Res 2004;29:212–215.
  24. Zheng Y, Ge S, Zhamg J, Guo Q, Ng M-H, Wang F, Xia N, Jiang Q: Swine as a principal reservoir of hepatitis E virus that infects humans in eastern China. J Infect Dis 2006;193:1643–1649.

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: September 27, 2006
Published online: January 26, 2007
Issue release date: April 2007

Number of Print Pages: 9
Number of Figures: 4
Number of Tables: 2

ISSN: 0300-5526 (Print)
eISSN: 1423-0100 (Online)

For additional information: https://www.karger.com/INT


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