Cellular Physiology and Biochemistry

Original Paper

Free Access

Localization and Thyroid Hormone Influenced Expression of Collagen II in Ovarian Tissue

Saha S.K.1 · Ghosh P.1 · Mitra D.1 · Mukherjee S.2 · Bhattacharya S.3 · Roy S.S.1

Author affiliations

1Molecular Endocrinology Laboratory, Indian Institute of Chemical Biology, Kolkata,2Department of Endocrinology, Post Graduate Institute of Medical Research and Hospital, Kolkata,3School of Life Sciences, Visva Bharati University, Santiniketan

Corresponding Author

Sib Sankar Roy

Molecular Endocrinology Laboratory, Indian Institute of Chemical Biology

4 Raja S. C. Mullick Road, Kolkata-700032 (India)

Tel. +91 33/2473-3491, Fax: +91 33/2473-5197

E-Mail sibsankar@iicb.res.in

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Cell Physiol Biochem 2007;19:067–076

Abstract

Collagen type II (Col II), one of the main components of the hyaline cartilage, is a member of the fibril-forming collagen family. Due to its amino acid composition, the extent of lysine hydroxylation of Col II is much higher than that of other fibril forming collagens. Since lysyl hydroxylase isoforms are less synthesized in hypothyroid ovarian tissue, Col II level is expected to be reduced here and contribute to the degradation of ovarian ECM in this condition. As there was no previous report, we have demonstrated Col II expression in rat ovary. Col2A1 mRNA shares significant part of the total collagens in ovary as shown by the relative expression of the major collagen genes present in this tissue. It has also been shown that Col II is down regulated in hypothyroid ovarian tissue and its expression is increased upon stimulation by thyroid hormone (T3). To know whether less Col II in hypothyroid ovarian tissue is due to less synthesis of the protein or its increased rate of degradation is also involved in it, we demonstrated the status of Collagen - degrading Matrix Metalloproteinases in this condition and found up regulation of MMP-1, -8 and -13 in hypothyroid rat ovary. The present study shows the reduced Col II expression in hypothyroid rat ovary, with the concomitant increase in Col II degradation. This information will be useful for further studies on reproductive disorders.

© 2007 S. Karger AG, Basel




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Article / Publication Details

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Abstract of Original Paper

Published online: January 16, 2007
Issue release date: January 2007

Number of Print Pages: 10
Number of Figures: 0
Number of Tables: 0

ISSN: 1015-8987 (Print)
eISSN: 1421-9778 (Online)

For additional information: https://www.karger.com/CPB


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