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Caries Res 2017;51:209-215
(DOI:10.1159/000458537)

Genetic Polymorphisms in DEFB1 and miRNA202 Are Involved in Salivary Human β-Defensin 1 Levels and Caries Experience in Children

Lips A.a · Antunes L.S.a, b · Antunes L.A.b · Abreu J.G.B.a · Barreiros D.c · Oliveira D.S.B.c · Batista A.C.a · Nelson-Filho P.c · Silva L.A.B.c · Silva R.A.B.c · Alves G.G.a · Küchler E.C.c

Author affiliations

aClinical Research Unit, Fluminense Federal University, Niterói, bDepartment of Specific Formation, School of Dentistry of Nova Friburgo, Fluminense Federal University, Nova Friburgo, and cDepartment of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

Corresponding Author

Dr. Erika Calvano Küchler and Dr. Gutemberg Gomes Alves

Department of Pediatric Dentistry

School of Dentistry of Ribeirão Preto, Av. do Café, S/N

Ribeirão Preto, SP 14040-904 (Brazil)

E-Mail erikacalvano@gmail.com

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Abstract

The antimicrobial peptides human β-defensins (hBDs) are encoded by β-defensin genes (DEFBs) and are possibly involved in caries susceptibility. In this study we aimed (1) to investigate the relationship between salivary hBDs and caries and (2) to evaluate the association of genetic polymorphisms in DEFB1 and microRNA202 (miRNA202) with salivary levels of hBDs and caries experience. Two data sets were available for this study, totalizing 678 Brazilian children. Dental examination and saliva collection were performed in all included children. The salivary level for hDB1, hBD2, and hBD4 was assessed by ELISA sandwich technique in 168 children. The DNA was extracted from saliva, and polymorphisms in DEFB1 and miRNA202 were analyzed by real-time PCR. Statistical analysis was performed to investigate the associations between caries experience, hBD salivary level, genotype, and allele distribution, with an alpha of 0.05. The hBD1 level was significantly higher in caries-free children (p < 0.0001). The miRNA202 was associated with a lower level of salivary hBD1 (p < 0.05). Also, the polymorphic distribution of miRNA202 was associated with caries (p = 0.006). The polymorphisms in DEFB1 were not associated with hBD salivary level and caries experience (p > 0.05). In conclusion, our results indicate that genetic polymorphism in miRNA202 is involved in hBD1 salivary level as well as caries experience in children.

© 2017 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: October 12, 2016
Accepted: January 30, 2017
Published online: March 25, 2017

Number of Print Pages: 7
Number of Figures: 1
Number of Tables: 5

ISSN: 0008-6568 (Print)
eISSN: 1421-976X (Online)

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