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J Vasc Res 2004;41:400–411

Fibered Confocal Fluorescence Microscopy (Cell-viZio™) Facilitates Extended Imaging in the Field of Microcirculation

A Comparison with Intravital Microscopy

Laemmel E.a · Genet M.b · Le Goualher G.b · Perchant A.b · Le Gargasson J.-F.c · Vicaut E.a

Author affiliations

aLaboratoire d’Etude de la Microcirculation, Université Paris 7, bMauna Kea Technologies, et cLaboratoire de Physiopathologie cellulaire et moléculaire de la Rétine, INSERM U592, Paris, France

Corresponding Author

Eric Vicaut, MD, PhD

Laboratoire d’Etude de la Microcirculation

10 avenue de Verdun

FR–75010 Paris (France)

Tel./Fax +33 1 40 05 49 73, E-Mail eric.vicaut@lrb.ap-hop-paris.fr

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This study investigated the capability of fibered confocal fluorescence microscopy (FCFM) to provide in vivo microvascular observations. FCFM is specifically designed for in vivo in situ observation thanks to a probe composed of a fiber bundle and micro-optics having a diameter as small as 650 µm. In the first part of the study, we compared the main characteristics of FCFM with those of intravital fluorescence microscopy (IFM). A mouse cremaster preparation was used as a common basis to allow for imaging with both modalities. We discussed the feasibility of obtaining quantitative measurements usually provided by IFM in the context of FCFM: morphometry, capillary permeability, functional capillary density, vasoconstriction and dilation effects. In addition, the possibility to visualize fluorescent red blood cells or leukocytes was also evaluated. Phototoxicity issues and limitations of FCFM were also discussed. We showed that FCFM allows observations and measurements usually provided by IFM and that the real-time capability of the system, as well as the flexibility and small diameter of the optical probe enable micro-invasiveness and can extend imaging capabilities for in vivo in situ observations when compared to IFM.

© 2004 S. Karger AG, Basel


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Article / Publication Details

First-Page Preview
Abstract of Research Paper

Received: June 24, 2004
Accepted: July 25, 2004
Published online: November 19, 2004

Number of Print Pages: 12
Number of Figures: 13
Number of Tables: 1

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: https://www.karger.com/JVR